Fig. 6: Diurnal lipidomics profiling corroborates the entrainment of fatty acid metabolism by DRF.
From: Multi-omics profiling reveals rhythmic liver function shaped by meal timing
a Histograms showing phase distribution of diurnal hepatic lipids and phase-shift distribution of dual-cycling hepatic lipids, the rhythmicity of which is determined by RAIN (adjusted P < 0.05), MetaCycle (adjusted P < 0.05) and Circacompare (P < 0.05) (n = 28 mice per group, n = 4 mice per time point). Two-sided multiple comparisons are adjusted using the default settings. Exact p-values are provided in the associated Source data file. Locked, phase shift [0, 2 h]; inverted, phase shift [8, 12 h]; numbers denote phase-locked/inverted rhythmic lipids over total dual-cycling lipids. b Diurnal profiles of dual-cycling lipids in mouse livers (n = 4 mice per time point). Average expression levels were log2-transformed and scaled. BMP, bis(monoacylglycero) phosphate; DAG, diacylglycerol; PA, phosphatidic acid; PE, phosphatidylethanolamine; PI, phosphatidylinositol. c Diurnal profiles of representative diurnal lipids in mouse liver. Data are presented as mean values  ±  standard error of the mean, n = 4 mice per time point for 7 time points. Two-sided unpaired Student’s t-tests with Bonferroni correction. d Class of diurnal hepatic lipids and 24-h levels of representative diurnal lipids from TRF mice. TAG, triacylglycerol; PS, phosphatidylserine; SM, sphingomyelin. e Diurnal profiles of diurnal N-acylphosphatidyl ethanolamine (NAPE), endocannabinoid/N-acylethanolamine (NAE) and monoacylglycerol (MAG) species in TRF mouse livers, the rhythmicity of which is determined by RAIN (P < 0.05), MetaCycle (P < 0.05), and Circacompare (P < 0.05). Data are presented as mean values  ±  standard error of the mean, n = 4 mice per time point for 7 time points. Two-sided unpaired Student’s t-tests with Bonferroni correction. Source data are provided as a Source data file.
