Fig. 2: Micropatterned gut spheroid generator (μGSG) enhances the biogenesis of different types of region-specific gut spheroids from human pluripotent stem cells.
From: Mechanically enhanced biogenesis of gut spheroids with instability-driven morphomechanics
a–c Induction of dAFG (dorsal anterior foregut) (a), vAFG (ventral anterior foregut) (b), and MHG (mid-hind gut) (c) spheroids (upper) and representative confocal micrographs (lower) showing the staining of indicated markers in spheroids derived from indicated conditions. Scale bar: 50 μm. n = 5 independent experiments. d Bar plot showing normalized number of dAFG, vAFG and MHG spheroids generated by monolayer-induction and μGSG, respectively, using iPS18 cell line. n = 3 independent experiments. e–g Scatter plots showing percentages of cells positive of HNF1β and SOX2 in dAFG (e), SOX2 and NKX2.1 in vAFG (f), and CDX2 in MHG (g) spheroids derived from indicated conditions. n = 5 independent experiments. Data were plotted as mean ± s.d. P-values were calculated using unpaired, two-sided Student’s t-test. Source data are provided as a Source Data file.
