Fig. 2: MS/MS of RvD1 do not match between standard and sample, and an extracted buffer blank shows absence of peak, but several detectable “diagnostic” ions for Mar1, with some being detected throughout the entire run. | Nature Communications

Fig. 2: MS/MS of RvD1 do not match between standard and sample, and an extracted buffer blank shows absence of peak, but several detectable “diagnostic” ions for Mar1, with some being detected throughout the entire run.

From: Failure to apply standard limit-of-detection or limit-of-quantitation criteria to specialized pro-resolving mediator analysis incorrectly characterizes their presence in biological samples

Fig. 2

A Screenshot of Supplementary Fig. 1A showing MS/MS of standard and sample, from Gomez et al. B MS/MS of an RvD1 standard generated in one of our laboratories. C Chromatogram, monitoring for Mar1 at m/z 359–250. D MS/MS at 10–10.2 min, where the Mar1 standard elutes, showing isolation and fragmentation of ion at m/z 359. E Zoomed in regions of centroid spectrum showing background ions contain several “diagnostic” ions for Mar1, as labelled by red arrows. F Ions at m/z 113 or 141 that are detected following fragmentation of m/z 359 are detected throughout the chromatographic run. Panel G. Figure showing first report of Mar1 MS/MS spectrum from12. G Reproduced from Serhan et al.12, the first report of maresin-1 (Mar1) as a novel metabolite of DHA.

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