Fig. 4: uORF-targeting ASOs regulate GATA4 protein expression and cardiomyocyte hypertrophy in human ESC-derived cardiomyocytes. | Nature Communications

Fig. 4: uORF-targeting ASOs regulate GATA4 protein expression and cardiomyocyte hypertrophy in human ESC-derived cardiomyocytes.

From: Secondary structures that regulate mRNA translation provide insights for ASO-mediated modulation of cardiac hypertrophy

Fig. 4

a Western blot analysis of GATA4 protein expression in human ESC-derived WT and homozygous ∆uORF CMs with ASO1 and ASO2 (50 nM) or control ASO treatment. b RT-qPCR measurement of GATA4 mRNA normalized to ACTB. c Representative images of α-Actinin (green) and NKX2-5 (red) immunostaining in addition to DAPI (blue) in ESC-derived CMs treated with control ASO, ASO1, or ASO2. Scale bar: 100 μm. Co-staining of α-actinin (green) and NKX2-5 (red) discerns CMs from mis-differentiated cells. Cell surface area was measured for five different clumps of cells as the total surface area was divided by the number of cells. d MYH6 mRNA expression in ESC-derived WT and homozygous ∆uORF CMs at baseline. e MYH6 mRNA expression in ESC-derived WT and homozygous ∆uORF CMs with ASO1 and ASO2 or control ASO (50 nM) treatment. Data are represented as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001; Statistical significance was confirmed by unpaired two-tailed Student t test for c and d, and one-way ANOVA followed by Holm–Sidak post hoc test for a, b, and e (N = 3 biological replicates). Source data are provided as a Source Data file.

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