Fig. 6: USP16 inhibits K48-linked ubiquitination and degradation of KEAP1, which are required for alleviation of hepatic IRI by FGF18. | Nature Communications

Fig. 6: USP16 inhibits K48-linked ubiquitination and degradation of KEAP1, which are required for alleviation of hepatic IRI by FGF18.

From: FGF18 alleviates hepatic ischemia-reperfusion injury via the USP16-mediated KEAP1/Nrf2 signaling pathway in male mice

Fig. 6

A The interaction of KEAP1 and USP16 in HepG2 was detected in H/R (4 h/6 h) challenge or not (n = 3). B The ubiquitination level of KEAP1 was determined in the indicated group in HepG2 cells (n = 3). C The ubiquitination level of KEAP1 was determined in the indicated group in HEK293 cells (n = 3). D HA-Ub or HA-Ub (K48-Mut) was transfected into HepG2 cells in the presence of si-USP16 or si-scramble. The ubiquitination level of KEAP1 was detected in the indicated group (n = 3). E Flag-USP16 overexpressed HepG2 were transfected with HA-KEAP1 (WT) or HA-KEAP1 (domain Mut). Relative protein expression by western blotting. F The ubiquitination level of KEAP1 was determined in the indicated group in HepG2 cells (n = 3). G HepG2 cells were subjected to H/R (4 h/6 h) challenge in the presence of FGF18. Then cells were simultaneously transfected Flag-USP16, Flag-USP16C205S, or not. Relative protein expression by western blotting (n = 3). The statistical significance of differences were assessed by two-tailed student unpaired t-test for (E). Other assays were assessed by one-way ANOVA, followed by Tukey’s multiple comparison test. Data are presented as means ± SEM with individual values. All numbers (n) are biologically independent experiments. Source data are provided as a Source Data file.

Back to article page