Table 2 Off-target analysis in rice T0 lines of Os-AG04 and Os-TG02 by GUIDE-seq and CRISPR-GE prediction

Index	Chromosome	Position	Method	Sequence	PAM	Gene_ID	Region	Edited
On-target	Chr03	4410363	–	AGTCCTGGCAAACAACCTGC	AGAAA	LOC_Os03g08570 (Os-AG04)	Intron	Yes
Off-target1	Chr08	17027595	GUIDE-seq	AGTACTCGGAATCAACATGC	TAAAG	LOC_Os08g27940	Intron	No
Off-target2	Chr03	22118232	GUIDE-seq	AGGCTTGGCGAAGTACCTGC	CGATG	–	Intergenic	No
Off-target3	Chr05	15939244	GUIDE-seq	AGTACTCGGAATCAACATGC	TAAAG	–	Intergenic	No
Off-target4	Chr02	32932893	GUIDE-seq	AGTCGTGGCTAACTAACTGG	AGGAT	–	Intergenic	No
Off-target5	Chr10	13417495	CRISPR-GE	AGTCATGGCAATCGACCAGC	AGAAA	LOC_Os10g25890	Intron	No
Off-target6	Chr04	7972534	CRISPR-GE	AGCACTGGAAAACACCCTGC	AGAAA	–	Intergenic	No
Off-target7	Chr06	9197794	CRISPR-GE	ATTCGTGACAAAAAAACTGC	AGAAA	–	Intergenic	No
Off-target8	Chr05	6095578	CRISPR-GE	AGTTCCGTCAAACAAACTTC	AGAAA	–	Intergenic	No
On-target	Chr03	4411032	–	TGGCATTTCTACCTTATCGA	TGAAA	LOC_Os03g08570 (Os-TG02)	Intron	Yes
Off-target1	Chr05	29520552	GUIDE-seq	TGGGATTGCTATTGTATCGA	CCGAC	LOC_Os05g51490	Intron	No
Off-target2	Chr05	19638874	GUIDE-seq	TGGCATGCCTACCTCCTCGT	CTCGT	LOC_Os05g33420	CDS	No
Off-target3	Chr08	589207	GUIDE-seq	TCTCATTTCGGCCTTATCGA	AAGGT	–	Intergenic	No
Off-target4	Chr08	24642717	GUIDE-seq	CTGAATTTCTTCCTTATCAA	GTCAA	LOC_Os08g38980	CDS	No
Off-target5	Chr03	27025699	GUIDE-seq	TGGAATTTGTACCATATCCA	TGAAT	LOC_Os03g47693	Intron	No
Off-target6	Chr03	24493324	CRISPR-GE	TGGCATTTATAGATTATTGA	AGAAA	–	Intergenic	No
Off-target7	Chr10	11655500	CRISPR-GE	TGAGAATCCTACCTTATCAA	AGAAA	LOC_Os10g22484	CDS	No
Off-target8	Chr05	1265737	CRISPR-GE	TTGAATTCCCACGTTATCGA	AGAAA	–	Intergenic	No

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