Fig. 2: Heated-inactivated B. adolescentis alleviated colon senescence phenotype by improving the intestinal barrier and restoring ISCs in G3 Terc -/- mice. | Nature Communications

Fig. 2: Heated-inactivated B. adolescentis alleviated colon senescence phenotype by improving the intestinal barrier and restoring ISCs in G3 Terc -/- mice.

From: Heat-inactivated Bifidobacterium adolescentis ameliorates colon senescence through Paneth-like-cell-mediated stem cell activation

Fig. 2

a The schematic diagram of the WT and G3 Terc-/- mice experimental procedure. b Representative image of gross morphology and length analysis of the mouse colon. n = 6 animals per group, the error bars indicate the mean ± SEM. Comparisons were performed by One-way ANOVA analysis followed by Dunnett’s multiple comparisons test. c Representative image of H&E staining and PAS staining, immunofluorescence image of Muc2, ZO-1 and Ocln and Lgr5, FISH probe of B. adolescentis in the colon from WT + PBS, G3 Terc-/- + PBS and G3 Terc-/- + B.a mice. Scale bar, 50 μm, n = 6 animals per group. d The mucosal height was measured. n = 15 random fields per group. Data were represented as mean ± SEM. Comparisons were performed One-way ANOVA analysis followed by Tukey’s multiple comparisons test. e Relative mRNA levels of ZO-1 and Ocln genes in WT + PBS, G3 Terc-/- + PBS and G3 Terc-/- + B.a mice. n = 6 animals per group. Data were represented as mean ± SEM. Comparisons were performed by Kruskal–Wallis test followed by Two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli. f Relative LPS levels in the fecal and serum samples in WT + PBS, G3 Terc-/- + PBS and G3 Terc-/- + B.a mice. n = 6 animals per group. Data were represented as mean ± SEM. Comparisons were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. g Relative mRNA levels of p53, p21 and Lgr5 gene in WT + PBS, G3 Terc-/- + PBS and G3 Terc-/- + B.a mice. n = 6 animals per group. Data were represented as mean ± SEM. Comparisons in p53, p21 were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test and in Lgr5 were performed by Kruskal–Wallis test followed by two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli. h The protein level of p53, p21 and Lgr5 were detected by immunoblot from WT + PBS, G3 Terc-/- + PBS and G3 Terc-/- + B.a mice. *p < 0.05, **p < 0.01, ***p < 0.001. Source data and exact p value are provided as a Source data file.

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