Fig. 5: Wnt/β-catenin pathway involved in heated-inactivated B. adolescentis induced ISCs proliferation.

a Heatmap visualization of a series of Wnt/β-catenin and Notch pathway genes in PBS and B. adolescentis treated colonoids. Asterisk denotes statistical significance. Source data and exact p value are provided as a Source data file. b Relative mRNA levels of Cyclin D1 and c-Myc were shown. n = 10 individual experiments per group. Data were represented as mean ± SEM. Comparisons were performed by unpaired, two-tailed t test. c Representative images of immunostaining staining with DAPI (blue) and active β-catenin (green) in colonoids. Scale bar, 25 μm. n = 3 independent experiments. d Immunoblot of Cyclin D1, c-Myc and active β-catenin in colonoids from PBS or B. adolescentis treated group. n = 3 independent experiments. Data were represented as mean ± SEM. Comparisons were performed by Two-way ANOVA followed by Two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli. e The expression of c-Myc, Cyclin D1 and Axin2 genes were detected by qPCR from WT + PBS, G3 Terc^-/- + PBS and G3 Terc^-/- + B.a mice group. n = 6 animals per group. Data were represented as mean ± SEM. Comparisons in Cyclin D1 and Axin2 were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test and in c-Myc were performed by Kruskal–Wallis test followed by Two-stage linear step-up procedure of Benjamini, Krieger and Yekutieli. test. f The protein level of c-Myc, Cyclin D were detected by immunoblot from WT + PBS, G3 Terc^-/- + PBS and G3 Terc^-/- + B.a mice group. n = 3 animals per group. *p < 0.05, **p < 0.01, ***p < 0.001. Source data and exact p value are provided as a Source data file.