Fig. 6: Wnt/β-catenin pathway involved in B. adolescentis-induced ISCs proliferation. | Nature Communications

Fig. 6: Wnt/β-catenin pathway involved in B. adolescentis-induced ISCs proliferation.

From: Heat-inactivated Bifidobacterium adolescentis ameliorates colon senescence through Paneth-like-cell-mediated stem cell activation

Fig. 6

a, b Representative images in organoid-forming capacity and size of colonoids quantified from PBS, B.a and IWR + B.a group. Scale bar, 100 μm. n = 3 independent experiments per group. Data were represented as mean ± SEM and represented relative to PBS control. Comparisons were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. c The expression of Lgr5 and Wnt/β-catenin target genes were detected by qPCR from PBS, B.a and IWR + B.a treated group. n = 3 independent experiments. Data were represented as mean ± SEM. Comparisons were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. d The protein level of Lgr5 and Wnt/β-catenin target genes were detected by immunoblot from PBS, B.a and IWR + B.a treated group. e Schematic diagrams of inhibition experiment in vivo from PBS, B.a, B.a + XAV group, n = 8 animals per group. f Representative images in organoid-forming capacity and size of colonoids quantified from PBS, B.a, B.a + XAV group on day 7. Arrows indicate crypt domains. Scale bar, 100 μm. Data was from 3 independent experiments with a total of n = 8 mice per group. Data were represented as mean ± SEM and represented relative to PBS control. Comparisons were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. g FITC-dextran concentration in the serum in PBS, B.a and B.a + XAV group mice. n = 8 animals per group. Data were represented as mean ± SEM. Comparisons were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. h Relative LPS levels in the fecal and serum samples in PBS, B.a and B.a + XAV mice. n = 8 animals per group. Data were represented as mean ± SEM. Comparisons were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. i Heatmap visualization of a series of (c-Myc, Cyclin D1, Lgr5, Ocln, ZO-1) genes in PBS, B.a and B.a + XAV group. Pound key (#) denotes statistical significance between PBS and B.a group, asterisk (*) denotes statistical significance between B.a and B.a + XAV group. n = 8 animals per group. Source data are provided as a Source Data file. Comparisons were performed by One-way ANOVA analysis followed by Tukey’s multiple comparisons test. j Representative image of AB-PAS staining and immunofluorescence image of active-β-catenin, ZO-1 and Ocln in the colon from indicated mice. n = 8 animals per group. Scale bar, 50 μm, n = 8 animals per group. *p < 0.05, **p < 0.01, ***p < 0.001. #p < 0.05, ##p < 0.01, ###p < 0.001. Source data and exact p-value are provided as a Source data file.

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