Fig. 1: Presence of NHI-plasma compromises cytoadherence of CS2 parasites to CSA. | Nature Communications

Fig. 1: Presence of NHI-plasma compromises cytoadherence of CS2 parasites to CSA.

From: Cryo-electron microscopy of IgM-VAR2CSA complex reveals IgM inhibits binding of Plasmodium falciparum to Chondroitin Sulfate A

Fig. 1

a Representative images from three experiments of CSA binding of CS2 IEs cultured in RPMI either supplemented with NHI-plasma (+IgM) or albumax (-IgM). The bound IEs were stained with acridine orange and visualized under confocal microscope at 20X under bright field and alexa 488 filter and the merged images of +IgM and -IgM are shown. Scale bar is 25 µm. b The box-whisker analysis of bound IEs from merged images in +IgM and -IgM and plotted as number of IEs bound/field. n = 3 performed in duplicates and 5 fields were counted per spot and represented as a point in the box-whisker plot, cross (x) represents the mean, line crossing the box plot is the median, lowest and highest whisker represents minimum and maximum data point, whereas lower bound of the box represents Quartile 1 and upper bound of the box represents Quartile 3, dot outside the whisker are outliers (original values are available in source data). P values were calculated using paired t-test and ***P < 0.0001. c A representative image from five experiments of Size exclusion chromatography of VAR2CSA and IgM-VAR2CSA complex resolved on superose6 3.2/300 and the chromatograms were superposed. Orange-VAR2CSA peak, blue-IgM-VAR2CSA complex. The fractions containing VAR2CSA were resolved on SDS-PAGE and coomassie stained d A representative image from three experiments of the IgM-VAR2CSA complex fractions obtained upon gradient fixation resolved on native-PAGE. * represents the fractions used for western blot using anti-VAR2CSA, anti-CH4 and J chain antibodies. These fractions were also used to prepare cryo-EM grids for data collection on 300kEV Titan Krios.

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