Fig. 1: Characterizing cell population diversification dynamics based on automated FC. | Nature Communications

Fig. 1: Characterizing cell population diversification dynamics based on automated FC.

From: Fitness cost associated with cell phenotypic switching drives population diversification dynamics and controllability

Fig. 1

a Chemostat culture is monitored based on automated FC. b The fluorescence distribution acquired by FC is assembled into a time scatter plot. This time scatter plot is then further reordered into 50 fluorescence bins in order to compute the evolution of the entropy H of the population (Supplementary Note 1). c The binned data are further processed by applying a gradient to compute the fluxes of cells into the phenotypic space, leading to the quantification of the total fluxes of cells per time interval F(t). Both H(t) and F(t) will be exploited for characterizing the phenotypic diversification dynamics of diverse cell populations. d Scheme of Segregostat set-up. Pulses of nutrients are added in function of the ratio between GFP negative and GFP positive cells, as recorded by automated FC. e Expected evolution of a Segregostat experiment where, upon controlled environmental forcing, several diversification cycles can be generated.

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