Fig. 3: Population dynamics for two cellular systems exhibiting bursts in diversification.
Temporal diversification profile for the PT7::GFP system in E. coli a and the PspoIIE::GFP system b cultivated in chemostat (from 0 to 40 h for PspoIIE::GFP) and Segregostat modes (from 40 to 100 h for PspoIIE::GFP). Below Segregostat cultivation modes, vertical lines indicate times of pulsing with the concentration (in g/L) displayed on the y-axis. Only the continuous phase of the cultivation is shown on the graphs. Bursts of diversification are highlighted in red on the fluorescence bins data. In both cases, the entropy H(t) and the fluxes of cells in the phenotypic space F(t) have been computed from the binned fluorescence data. The reproducibility of the data has been assessed based on two biological replicates (n = 2) for each system. For each time interval, 20,000 cells have been analyzed by FC (Supplementary Note 1 and Supplementary Fig. 3). The color bars represent the number of cells for the fluorescence time scatter plots and the number of cells per minute for the F(t) scatter plots. Source data are provided as a Source Data file.
