Fig. 2: Light and calcium response of opto-E-cad in suspension culture.
From: Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
a Schematic representation (upper panels) and bright field images (lower panels) of opto-E-cad-MDA cells (5 × 104 cells/ml) in suspension culture in the dark and under blue light incubated for 2 h at 30 rpm. In the dark, the cells form large aggregates but under blue light illumination remain as single cells. Scale bar is 50 μm. b Quantification of average cluster area, from left to right n = 35, 32, 18, 16, 12, 7 samples examined over at least 3 independent experiments. Boxplots show median, 25th, and 75th percentile. The lower and upper boundaries of whiskers indicate the minima and maxima, respectively. All comparisons are performed using Fisher’s One Way ANOVA test and p < 0.05 was treated as the significance threshold. c Quantification of average cell cluster area as a function of Ca2+ (two-tailed t-test, n = 6). Data are represented as mean values ± SD. Source data are provided as a Source Data file.
