Fig. 3: Temporal and bidirectional control over cell–cell adhesions.
From: Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
a Opto-E-cad allows to turn adhesions on upon placing cells in the dark and off upon blue light illumination. b Quantification of average cell cluster area over time in suspension culture in the dark and under blue light (n = 6). c Bright field images of opto-E-cad-MDA cells in suspension culture kept for 60 min under blue light and subsequently in the dark or blue light and formation kinetics of the cell–cell adhesions (n = 4). d Bright field images of opto-E-cad-MDA cells in suspension culture kept for 60 min in the dark and subsequently under blue light and reversion kinetics of cell–cell adhesions under blue light (n = 8). e Bright field images of opto-E-cad-MDA cells in suspension culture under repeated 60 min dark/blue light cycles and their clustering dynamics (n = 3). Data are represented as mean values ± SD. Scale bars are 200 μm. Source data are provided as a Source Data file.
