Fig. 5: Optogenetic control of collective cell migration and cell invasion.
From: Reversible photoregulation of cell-cell adhesions with opto-E-cadherin
a Bright field and vector field images of opto-E-cad-MDA cells in a wound healing assay in dark and under blue light for 16 h. Scale bar is 100 μm. 6 individual experiments were conducted. b Migration rate of the cell front for opto-E-cad-MDA cells (a) (n = 33). All comparisons are performed using Fisher’s One Way ANOVA test and p < 0.05 was treated as the significance threshold. c Velocity correlation lengths of opto-E-cad-MDA and MDA-MB-231 cells in the dark and under blue light, from left to right n = 64, 64, 93, 93 (two-tailed t-test). d Migration angle of velocity vectors of opto-E-cad-MDA in dark (upper liner) and under blue light (lower line). e Transwell invasion assay (two-tailed t-test, n = 4). Boxplots (b, e) show median, 25th, and 75th percentile. The lower and upper boundaries of whiskers indicate the minima and maxima, respectively. Source data are provided as a Source Data file.
