Fig. 3: SEKI and CRCA livers show structural and proteomic changes, accompanied by overproduction of acute-phase proteins.
From: Decreased liver B vitamin-related enzymes as a metabolic hallmark of cancer cachexia
a Representative transmission electron microscopic (EM) images of hepatocytes. CNT, control nude mouse; M, mitochondria; LD, lipid droplet. Bars, 1 μm. CNT, N = 2 mice; MEWO, N = 1 mouse; LNCA, N = 1 mouse; SEKI, N = 1 mouse; CRCA, N = 1 mouse; STRV, N = 1 mouse. b Enlarged views of CNT and SEKI showing rough endoplasmic reticula (ER). Red asterisk, expanded ER lumen. Bars, 250 nm. c Quantified morphological changes. Mitochondrial section, mitochondrial cross section area. Total lipid droplet area, total lipid droplet area of one imaging area (area size, 581.92 μm2) Expanded ER, percentage of image fields with expanded ER. CNT, EM images from 5 control mice. CNT, N = 5 mice; MEWO, N = 1 mouse; LNCA, N = 1 mouse; SEKI, N = 1 mouse; CRCA, N = 1 mouse; STRV, N = 1 mouse. Boxplot style, the middle line (median), the upper hinge (Q3, the third quantile), the lower hinge (Q1, the first quantile), the upper whisker (1.5 × (Q3 – Q1)), the lower whisker (1.5 × (Q3 − Q1)). Details of electron micrograph quantification are described in the Methods section (Statistics and Reproducibility). d Keyword-based volcano plots of liver proteomic data. MEWO, N = 3 mice; LNCA, N = 3 mice; SEKI, N = 3 mice; CRCA, N = 3 mice; STRV, N = 2 mice. Horizontal dashed lines show where P = 0.05 (Welch’s 2-sided t-test). APPs, acute-phase proteins. Plots are limited to a range of −1 to 1 on the X-axis and 0 to 5 on the Y-axis. Source data are provided as a Source Data file.
