Fig. 5: The impact of age on endometrial gene expression.
From: A molecular staging model for accurately dating the endometrial biopsy
Figure 5a. Two examples from the 60 endometrial genes that change expression significantly with increasing age. Statistical analysis used two-sided empirical Bayes moderated t-tests implemented in limma35 with corrections for multiple comparisons performed using the Benjamini-Hochberg method (ENSG00000180543: t = 6.025, p = 5.82e-9, padj = 5.84e-5; ENSG00000164778: t = −5.886, p = 1.29e-8, padj = 8.22e-5). Expression data were plotted following normalisation for changing expression across the menstrual cycle (n = 266 RNA-seq samples, 87 samples from GSE14154920). Figure 5b. Expression data for 2 genes plotted separately for menstrual, proliferative, and secretory samples (n = 266). Statistical analysis used two-sided empirical Bayes moderated t-tests implemented in limma with corrections for multiple comparisons performed using the Benjamini-Hochberg method (secretory phase ENSG00000147113: t = −5.074, p = 1.43e-6, padj = 0.010; secretory phase ENSG00000174175: t = −4.429, p = 2.09e-5, padj = 0.013). Source data are provided as a Source Data file.
