Fig. 6: Changing gene expression across the cycle.
From: A molecular staging model for accurately dating the endometrial biopsy
Figure 6a. Genes from RNA-seq analysis that significantly change expression (Padj <0.05) over 3.4% of the cycle (approximately equal to a 24-hour window) at different stages of the menstrual cycle. Statistical analysis used two-sided empirical Bayes moderated t-tests, tested relative to a threshold fold change of 1.2, implemented in limma35 with corrections for multiple comparisons performed using the Benjamini-Hochberg method. Using adjusted P values, 488 unique genes significantly change expression during menstruation, 44 during the proliferative phase, and 2921 during the secretory phase. Peak times of rapid change in gene expression approximately correspond to menstrual (3% of the way through the cycle), late proliferative (51%), POD3 (66%), POD5 (71%), POD11 (94%) and POD13 (98%). Figure 6b. Examples of 12 genes that change expression significantly at different times across the cycle. Source data are provided as a Source Data file.
