Fig. 6: The C-terminus controls intrinsic diffusion of Rad52 on ssDNA.
From: Yeast Rad52 is a homodecamer and possesses BRCA2-like bipartite Rad51 binding modes
Single-molecule FRET distributions of A (dT)40 overhang-ssDNA alone or (dT)40 overhang bound to B Rad52, or C Rad52ΔC. D, E HMM (hidden Markov model) analyzed FRET state distributions of smFRET traces. Data show Rad52 preferentially residing in the low FRET states. In contrast, Rad52ΔC readily accesses and resides in the higher FRET states. The dotted blue lines denote the four observed states. Source data are provided as a Source data file.
