Fig. 6: Acute and chronic GCR exposure abolished K+-evoked DA response in PFC, but not other neurotransmitters. | Nature Communications

Fig. 6: Acute and chronic GCR exposure abolished K+-evoked DA response in PFC, but not other neurotransmitters.

From: Complex 33-beam simulated galactic cosmic radiation exposure impacts cognitive function and prefrontal cortex neurotransmitter networks in male mice

Fig. 6

Time course of the effects of K+-evoked increases in extracellular levels of DA (a), 5-HT (b), NE (c), Glu (d), or GABA (e) in the PFC of control (n = 10), acute (n = 10), or chronic (n = 8) GCR exposed mice as a % basal (nM) levels. Dialysate samples were taken from the PFC every 20 min. Each dotted line indicates time points at which K+ concentration was increased. Ordinates, percentage of basal DA, 5-HT, NE, Glu, or GABA levels; abscissae, time in minutes during K+ stimulation. Each point indicates the mean (±S.E.M.) effect shown as of percentage of basal DA, 5-HT, NE, Glu, or GABA levels; neurotransmitter levels were uncorrected for probe recovery. Time course data were analyzed using a two-way ANOVA (treatment group and concentration and treatment group and time as factors, respectively) for repeated measures over concentration and time; overall changes from basal levels determined at 4 mM K+ perfusate solution were subjected to Tukey post hoc analyses. Analysis of these data showed: (a) for DA: main-effect treatment, F(2,325) = 8.41, P = 0.0003; main-effect time F(12,325) = 1.58, P > 0.05; a significant treatment × concentration interaction, F(24,325) = 0.81, P > 0.05; (b) for 5-HT: non-significant main-effect treatment, F(2,325) = 1.62, P > 0.05; significant main-effect time, F(12,325) = 3.93, P = 0.0001; non-significant treatment × concentration interaction, F(24,325) = 0.62, P = 0.92; (c) for NE: main-effect treatment, F values(2,325) = 7.91, P = 0.0004; main-effect time, F values(12,325) ≥ 2.40, P values = 0.005; non-significant treatment x concentration interaction, F(24,325) = 0.41, P = 0.99; and (d-e) for Glu and GABA: main-effect treatment, F values(2,325) ≥ 5.06, P values ≤ 0.007; main-effect time, F values (12,325) ≥ 6.23, P values < 0.0001; significant treatment x concentration interaction for GABA, F(24,325) = 2.74, P < 0001 but not Glu, F(24,325) = 0.91, P > 0.05. *P < 0.05 represents significant difference compared to basal values at 4 mM at time = 0. #P < 0.05 represents a significant difference compared to controls at each time point. $P < 0.05 represents significant differences between acute and chronic GCR-treated mice at each time point. DA dopamine, 5-HT serotonin, NE norepinephrine, Glu glutamate, GABA γ-aminobutyric acid.

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