Fig. 4: Depletion of SUN1/2 promotes chromatin openness at Il6 and Il1b loci.

a FISH analysis in WT and Sun1/2^DKO PEMs by using Chromosome 1 and 5 probes (n = 28 cells per group). Scale bar, 5 μm. b 3 C assay to detect interaction levels (relative positioning) between sites from IL1A and IL1B in the THP-1-derived macrophages (n = 3 biological replications per group). c FAIRE assay to detect accessibility and openness of chromatin at Il6 and Il1b loci in in WT and Sun1/2^DKO PEMs. d ChIP assay to analyze the enrichment of Pol II and H3K4me3 on Il6 and Il1b loci in WT and Sun1/2^DKO PEMs. e H3K4me2 and H3K4me3 staining in WT and Sun1/2^DKO PEMs. Scale bar, 5 μm. f ChIP-Seq of H3K4me3 in WT and Sun1/2^DKO PEMs. Heat maps showing the distributions of H3K4me3 within 10 kb of the TSSs. Composite H3K4me3 distribution profiles within 10 kb of the TSSs. ChIP assays were performed with anti- H3K4me3 antibodies in PEMs, followed by ChIP-Seq. *p < 0.05; **p < 0.01; ***p < 0.001 in comparison with control group. Two-sided Tukey post-hoc test was used to compared differences between groups after One-way ANOVA (a, b). Representative of 2 independent experiments (e). See also Supplementary Fig. 4.