Fig. 1: Ablation of tuft cells results in reduced ILC2s in both WT and HDTmx treated mice.

a Schematic for experimental SPEM induction and tuft cell ablation. 16-week-old BAC(Dclk1::CreERT2); Rosa26^DTA/+ (TC^Δ) or CreERT2-negative Rosa26^DTA (TC^WT) mice were treated with either HDTmx (250 mg/kg) once daily for 3 consecutive days to induce gastric spasmolytic polypeptide-expressing metaplasia (SPEM) and tuft cell ablation (in TC^Δ mice), or LDTmx (50 mg/kg) once daily for 3 consecutive days to induce tuft cell ablation (in BAC(Dclk1::CreERT2);Rosa26^DTA/+ mice). EP = endpoint. Created with BioRender.com. b Representative Immunofluorescence staining and quantification of stomachs from TC^WT and TC^Δ mice following treatments as described in Fig. 1a and enumerated for Gastric intrinsic factor (GIF)/GSII-lectin positive and TFF2-positive SPEM cells. N = 5, 6 and 6 respectively. Scale bar = 200μm. (c) Flow-cytometry quantification of SiglecF⁺CD24⁺EpCAM⁺ tuft cells from stomachs of TC^WT and TC^Δ mice following treatment with LDTmx or HDTmx. N = 7, 9, 7 and 7 respectively. d Flow-cytometry quantification of KLRG1⁺CD90.2⁺Lineage^-CD45⁺ ILC2s in stomachs of TC^WT and TC^Δ mice following treatment with LDTmx or HDTmx. N = 7, 5, 6 and 7 respectively. e Flow-cytometry quantification of ST2^-KLRG1⁺CD90.2⁺Lineage^-CD45⁺ ILC2s in stomachs of TC^WT and TC^Δ mice following treatment with LDTmx or HDTmx. N = 7, 5, 6 and 7 respectively. f Flow-cytometry quantification of ST2⁺KLRG1⁺CD90.2⁺Lineage^-CD45⁺ ILC2s in stomachs of TC^WT and TC^Δ mice following treatment with LDTmx or HDTmx. N = 7, 5, 6 and 7 respectively. Data represents mean ± SEM, p values from one-way ANOVA and Tukey’s multiple comparisons tests **p < 0.01, ***p < 0.001, ****p < 0.0001, ns - not significant. Each symbol represents an individual mouse. Data is from two pooled experiments. Source data and exact p values are provided as a Source Data file.