Fig. 3: Tuft cells and ILC2s promote tumor growth via IL25 and IL13 signaling. | Nature Communications

Fig. 3: Tuft cells and ILC2s promote tumor growth via IL25 and IL13 signaling.

From: A tuft cell - ILC2 signaling circuit provides therapeutic targets to inhibit gastric metaplasia and tumor development

Fig. 3

a t-SNE plot representing cell populations identified through unbiased cell clustering of pooled CD45.2+ and EpCAM+ cells sorted from gastric tissue and tumors of gp130+/+ (n = 10) and gp130F/F (n = 10) mice. b Cell clusters identified as tuft cells (purple) or ILC2s (red) using SingleR against the ImmGene database. c Violin plots displaying the distribution and expression of Il13, Il13ra1, Il4ra, Il25, and Il17rb across immune and epithelial cell populations of gp130+/+ and gp130F/F mice. d qRT-PCR analysis of gp130+/+ and gp130F/F sorted EpCAM+ and tuft cells (SiglecF+CD24+) for the expression of tuft cell genes Dclk1, Il25 and Il13ra1 (n = 6 mice). e qRT-PCR analysis of gp130+/+ and gp130F/F sorted CD45+ and ILC2s for the expression of Gata3, Il13, and Il17rb (n = 6 mice). Data represents mean ± SEM, p values from two-sided Student’s t-test or one-way ANOVA and Tukey’s multiple comparisons tests *p < 0.05, **p < 0.01, ***p < 0.001, ns - not significant. Source data and exact p values are provided as a Source Data file.

Back to article page