Fig. 6: Nitric oxide is required to clear C. violaceum in the granuloma.
From: An innate granuloma eradicates an environmental pathogen using Gsdmd and Nos2
a–f Mice were infected with 104 CFU WT C. violaceum. Dashed line, limit of detection; solid line, median. a Survival analysis of WT and Nos2–/– mice. Representative of 2 experiments, each with 11 mice per genotype. ****p < 0.0001, by Kaplan–Meier survival analysis. b Bacterial burdens in the liver and spleen at 3 and 5 dpi. Representative of 2 experiments; each point is a single mouse, 3–4 mice per day per genotype. ns, not significant, ***p = 0.0007, ****p < 0.0001, by two-way ANOVA. c Bacterial burdens in the liver and spleen at 7 dpi. Combined 2 experiments, each point is a single mouse. N = 10 (WT) or 9 (Nos2–/–) mice. **p = 0.0076 by Mann–Whitney and ***p = 0.0005 by unpaired two-tailed t test. d Serial sections of WT and Nos2–/– livers stained by H&E or indicated IHC markers 5 dpi. Representative of two experiments, each with 3–4 mice per genotype, each with multiple granulomas per section. e Flow cytometry of total neutrophil and total macrophage numbers from WT and Nos2–/– livers 3 and 7 dpi. Data combined from two experiments. ns, not significant, *p = 0.0114, and ****p < 0.0001, by two-way ANOVA. f Spatial transcription of Nos2 expression within WT granulomas at indicated timepoints. One liver was harvested per the indicated timepoint for spatial transcriptomic analysis.
