Fig. 1: DSS accelerated tumorigenesis and shortened lifespan. | Nature Communications

Fig. 1: DSS accelerated tumorigenesis and shortened lifespan.

From: Sleeping Beauty transposon mutagenesis identified genes and pathways involved in inflammation-associated colon tumor development

Fig. 1

a The alleles used for each SB mutagenesis screening and abbreviations for each mouse group. b Tamoxifen (20 mg/ml) was administered intraperitoneally (i.p.) three times to activate Cre at 4 weeks of age, and then 2.5% DSS in drinking water was administered three times for 5 days to induce chronic inflammation in the colon. c Survival of mice was significantly shortened by DSS treatment. The average age of K-SB (45.5 weeks), K-SB + DSS (32.4), KT-SB (46.4), KT-SB + DSS (35.2), KP-SB (38.0) and KP-SB + DSS (31.3) mice. Log-rank test. d The number of colonic tumors/mouse for each screen. Data are represented as mean values +/− SD. Two-sided t test. e–i HE staining for representative colon tumors of K-SB + DSS mice (e, f), KT-SB + DSS mice (g–i). A High-magnification image of the inset in (e) is (f). High-magnification images of the insets in (g) are (h) and (i). Scale bars, 500 µm (e, g) and 100 µm (f, h, i). j Pie charts showing the percentage of adenomas (A), adenocarcinomas (AC), and metastatic tumors (Mx).

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