Fig. 1: Schematic representation of Melac-Chip to capture the in vivo dynamics of newly-produced EVs during immunotherapy. | Nature Communications

Fig. 1: Schematic representation of Melac-Chip to capture the in vivo dynamics of newly-produced EVs during immunotherapy.

From: Capturing nascent extracellular vesicles by metabolic glycan labeling-assisted microfluidics

Fig. 1

Tumor-bearing mice were simultaneously treated with PD-L1 antibody and Ac4ManNAz (tetraacetylated N-Azidoacetyl-mannosamine), an unnatural sugar for metabolic labeling of EVs with azido groups. Subsequently, the azido-labeled EVs were tagged with biotin groups by click chemistry and captured by streptavidin (SA)-modified herringbone microfluidic chip.

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