Fig. 4: ROS accumulated in ospus1-1 and the accumulation was suppressed in suppressor mutants. | Nature Communications

Fig. 4: ROS accumulated in ospus1-1 and the accumulation was suppressed in suppressor mutants.

From: A mitochondrial pentatricopeptide repeat protein enhances cold tolerance by modulating mitochondrial superoxide in rice

Fig. 4

a NBT and DAB staining were used to assess the accumulation of O2•- and H2O2, respectively. Scale bars, 1 cm. b Schematic drawing of relationship between albino phenotype and ROS accumulation pattern in ospus1-1 at 22 °C. The albino occurred in the whole leaf blade which is consistent with O2•- accumulation pattern. c In-gel activity assay of various SOD isozymes in seedlings of wild type, mutants, suppressor mutants, and complemented plants grown at 28 °C or 22 °C. Equal amounts (20 µg) of total protein were subjected to native-PAGE to separate different SOD isozymes. Experiments were repeated three times independently with similar results. Source data for (a, c) are provided as a Source Data file.

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