Fig. 5: Overexpression of SOD genes suppresses ROS accumulation and the albino phenotype in ospus1-1. | Nature Communications

Fig. 5: Overexpression of SOD genes suppresses ROS accumulation and the albino phenotype in ospus1-1.

From: A mitochondrial pentatricopeptide repeat protein enhances cold tolerance by modulating mitochondrial superoxide in rice

Fig. 5

a Relative expression levels of Mn-SOD (LOC_Os05g25850), Cu/Zn-SOD (LOC_Os07g46990) and Fe-SOD (LOC_Os06g05110) genes in 9311, ospus1-1 mutant, and the SOD overexpression plants grown at 28 °C or 22 °C. Values are means ± S.D. (n = 6) of three biological replicates. Differences between ospus1-1 mutant and SOD overexpression lines grown under the same conditions are determined by unpaired Student’s t-test (two-tailed). Exact P values are shown. b Phenotypic responses to temperature (28 °C or 22 °C) of the 9311, ospus1-1, and ospus1-1 transgenic plants overexpressing Mn-SOD (LOC_Os05g25850), Fe-SOD (LOC_Os06g05110), or Cu/Zn-SOD (LOC_Os07g46990). Scale bars, 5 cm. c Accumulated ROS in ospus1-1 was suppressed by overexpressing SOD-encoding genes. The accumulation of O2•- and H2O2 in the leaf of rice grown at 22 °C in (b) were assessed by NBT and DAB staining, respectively. The numbers on top of each photo show how often this representative staining pattern occurred, relative to total examined pictures. Scale bars, 1 cm. Source data for (a–c) are provided as a Source Data file.

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