Fig. 6: Assessment of cold stress tolerance of sop10 single mutant plants at seedling stage.
a Seedlings of LKZ1, ZJZ17, ZZ35, and their two corresponding sop10CR mutants were grown at 28 °C for 2 weeks, shifted to 8 °C for 2 days, and allowed to recover at 28 °C for 4 days. Scale bars, 8 cm. b Survival rates of LKZ1, ZJZ17, ZZ35, and their sop10CR seedlings after cold treatment in (a). Values are means ± S.D. (n = 50 individual plants) of three biological replicates. Differences between mutants and wild type grown under the same conditions are determined by unpaired Student’s t-test (two-tailed). Exact P-values are shown. c NBT and DAB staining were used to assess the accumulation of O2•- and H2O2 in LKZ1, ZJZ17, ZZ35, and their sop10CR mutant seedlings before and after cold treatment in (a). The numbers on top of each photo show how often this representative staining pattern occurred, relative to total examined pictures. Scale bars, 1 cm. d In-gel assay of NADH oxidase capacity of LKZ1, ZJZ17, ZZ35, and their sop10CR mutants. Dihydrolipoamide dehydrogenase activity was used as a loading control. The red triangle indicates mitochondrial complex I, and the black triangle indicates dihydrolipoamide. Experiments were repeated two times independently with similar results. Source data for (b–d) are provided as a Source Data file. Experiments were repeated three times with similar results.
