Fig. 5: Alkbh5 loss induces RNA accumulation and a consequent overtranslation in oocytes.
a Scatterplots comparing the relative expression changes from the GV to MII stages in WT and Alkbh5−/− samples respectively. Significantly up- and downregulated genes are highlighted (|log2[fold change (MII/GV)]| > 1, q < 0.05). b Venn diagram of maternal decayed genes (fold change (MII/GV) < 0.5, q < 0.05) compared between WT and Alkbh5−/− oocytes. c GO analysis of maternal transcripts that are degraded in WT oocytes, but are stabilized in Alkbh5−/− oocytes, as indicated in (b). The P values are calculated using hypergeometric test of functional annotation in DAVID website with default parameters. The top 5 categories are reported. d Comparisons between degradation rates of representative RNAs in WT and Alkbh5−/− oocytes during GV-MII transition. The mRNA levels are normalized to Actb. The data are presented as mean ± SD. P value, two-tailed Student’s t test. n = 3 biological replicates. e PAT analysis showing the changes in poly(A)-tail length in WT and Alkbh5−/− oocytes during meiotic maturation. Poly, polyadenylated; De deadenylated. f Representative images of global translation activity in WT and Alkbh5−/− oocytes by culture in the presence of HPG. The abnormal aggregated foci are indicated (right panel; yellow arrowhead). Extruded polar bodies are highlighted. W/O, without; PB1, first polar body. g Quantifications of HPG intensity in (f). An average fluorescence intensity is estimated by single oocyte measurement and is plotted as a single dot. The mean and SD are from three or more repeated recordings. P value, two-tailed Student’s t test. W/O PB vs. WT, P = 0.000115. n = 22 (WT), 7 (KO with PB) and 22 (KO without PB) oocytes are examined. Source data are provided as a Source Data file.
