Fig. 6: The m⁶A landscape of mouse oocyte upon Alkbh5 depletion.

a Schematic illustration showing the pipeline of m⁶A meRIP-seq as detailed in “Methods”. In brief, about 2800 GV oocytes are collected from WT and Alkbh5^−/− mice after PMSG injection, and are prepared for m⁶A methylome analysis. b Examples of m⁶A-specific motifs detected from WT and Alkbh5^−/− samples. The consensus motif of m⁶A “RRACH” (R = G or A; H = A, C, or U) observed in the m⁶A-seq are illustrated along with P values analyzed by HOMER. c The m⁶A-peak distribution of Alkbh5^−/− samples compared with the controls. d Heatmap showing differential hyper- and hypo- m⁶A peaks (hyper, DiffModLog₂Fc > 1 with P < 0.01; hypo, DiffModLog₂Fc < −1 with P < 0.01) after Alkbh5 deletion. Each m⁶A peak is color-coded from blue to red according to their DiffModLog₂Fc values. P values are calculated based on the Poisson distribution. e Representative IGV diagram of m⁶A distributions of the hypermethylated gene Setd6. f GO analysis of genes containing differentially changed m⁶A peaks following Alkbh5 knockout. Functions of interest are annotated and numbers of genes enriched are also labeled. The enrichments and P values are calculated using hypergeometric test of functional annotation in DAVID website with default parameters. g Cumulative fraction showing the difference in degradation rates (log₂[fold change (MII/GV)]) between the m⁶A-modified and unmodified RNAs in WT oocytes. P values, one-sided Kolmogorov-Smirnov test. h Cumulative fraction showing the degradation rates (log₂[fold change (MII/GV)]) of m⁶A-modified RNAs in WT and Alkbh5^−/− oocytes, respectively. P values, one-sided Kolmogorov–Smirnov test. i Cumulative fraction showing the degradation rates (log₂[fold change (MII/GV)]) of unmodified RNAs in WT and Alkbh5^−/− oocytes, respectively. P values, one-sided Kolmogorov-Smirnov test. j Violin plot showing the expressional levels of m⁶A-modified transcripts in WT and Alkbh5^−/− oocytes at MII stage. The data are presented as violin with central line denoting mean value. P values, two-tailed Mann-Whitney test. Source data are provided as a Source Data file.