Fig. 5: Optogenetic inactivation of the RE selectively impairs extinction memory retrieval.
From: Thalamic nucleus reuniens coordinates prefrontal-hippocampal synchrony to suppress extinguished fear
A Illustration depicting fiber placement and intracranial injections of either the active Jaws (AAV8-CaMKII-Jaws-GFP) or GFP control virus (AAV8-CaMKII-GFP) into the RE. B Experimental timeline. Average freezing behavior during fear conditioning (C), context exposure (D), and extinction (E). F Average freezing behavior during extinction retrieval showing that optogenetic inhibition of the RE selectively increases freezing in conditioned animals expressing the active Jaws virus (n = 12; 5 male and 7 female; p = 0.0044, two-way ANOVA with Bonferroni correction), but not GFP (n = 10; 6 male and 4 female; p = 0.969) or non-conditioned control animals (n = 8; 5 male and 3 female; p > 0.999). RE nucleus reuniens of the thalamus, GFP green fluorescent protein. Line plots represent mean ± s.e.m.s. **p < 0.01. Source data are provided as a Source Data file.
