Fig. 2: FHL1 facilitates CHIKV disease development and viral replication in mice.
WT and FHL1−/− mice were infected with CHIKV at 104 PFU or mock-infected with PBS. Disease was monitored daily and assessed by measuring the height and width of the perimetatarsal area of the ipsilateral hind foot (a). The data shown are representative of two independent experiments; n = 10 mice per group (***P < 0.001; ****P < 0.0001; two-way ANOVA with the Bonferroni posttest). Serum was collected at 1, 3 and 5 dpi and processed for plaque assays (b). Dots represent individual animals (n = 9). The data shown are representative of two independent experiments. Data are presented as box and whisker ± SD with the mean indicated by a line across the box, maximum to minimum points (*P < 0.05; **P < 0.01; ***P < 0.001; Mann–Whitney test). The contralateral and ipsilateral quadriceps and ankles were harvested at 3 and 7 dpi. Viral titers were determined by a plaque assay (c–f). Dots represent individual animals (n = 9). The data shown are representative of two independent experiments. Data are presented as box and whisker ± SD with the mean indicated by a line across the box, maximum to minimum points (*P < 0.05; ***P < 0.001; ****P < 0.0001; Mann–Whitney test). Source data are provided as a Source Data file.
