Fig. 8: Inhibition of NOTCH signaling in rhabdoid cell lines.

A Western blot analyses for Notch intracellular domain NICD in CHLA-02 and IC-032 cell lines treated with DAPT NOTCH inhibitor or DMSO as a control. The experiment has been repeated 2 times for CHLA-02. B, C Volcano plot showing the differential gene expression analysis results of DAPT treatment versus DMSO in CHLA-02 (B) and in IC-032 (C) cell lines. The X axis indicates the log₂ transformed fold-change and the Y axis indicates the reverse of the log₁₀ transformed adjusted p-value. The number of significantly repressed and overexpressed genes are labeled in blue and in red, respectively. Genes having an absolute fold-change higher than 2 and an adjusted p-value lower than 0.05 are colored either in blue (for genes repressed in DAPT-treated cells) or in red (for genes overexpressed in DAPT-treated cells). The dotted green horizontal and vertical lines correspond to a p-value = 0.05 and to absolute fold-change = 2, respectively. Negative binomial GLM and Wald test were applied for gene expression comparison and generated p-values were corrected using the Benjamini and Hochberg method. D, E Top ten significantly enriched Gene Ontology (Biological Processes only) gene sets in genes that are differentially repressed (D) or overexpressed (E) in DAPT-treated CHLA-02 cells compared with DMSO. F, G Top ten significantly enriched Gene Ontology (Biological Processes only) gene sets in genes that are differentially repressed (F) or overexpressed (G) in DAPT-treated IC-032 cells compared with DMSO. H, I Heatmap showing the level of expression of a selection of interested genes in DAPT-treated cells compared with DMSO in CHLA-02 (H) and in IC-032 (I) cell lines. Color codes at the left and at the top of the heatmaps are shown below the figure. Source data are provided as a Source Data file.