Table 1 Main contacts that stabilize the back-folded conformation

From: How myosin VI traps its off-state, is activated and dimerizes

Head

Tail

Motor state

KD (nM)

∆G° (kcal/mol)

n

MDIns2/IQ/3HB

YFPCBD

ADP.VO4

144 ± 61

−9.3

3

MDIns2

YFPCBD

ADP.VO4

343 ± 197

−8.8

3

MD

YFPCBD

ADP.VO4

3920 ± 1453

−7.4

4

Ins2/IQ/3HB

YFPCBD

-

250 ± 86

−9.0

4

MDIns2/IQ/3HB

YFPCBD

NF

726 ± 480

−8.4

2

MDIns2/IQ/3HB

YFPCBDD1157V.Y1159D.D1161R.Q1163V

ADP.VO4

n.b.

-

3

  1. Dissociation constant (KD) ± KD confidence (with a 68% confidence using the NTAnalysis software) determined by microscale thermophoresis of Myo6 Head constructs against Myo6 Tail constructs (constructs schematized in Fig. 1a). Standard Gibbs free energy was obtained from the KD values, using the quantitative relationship ∆G = RTln(KD). Microscale thermophoresis profiles are presented in Supplementary Fig. 6. Source data are provided as a Source Data file.
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