Fig. 2: Distinct roles for ClpP and ClpX in SOS response induction. | Nature Communications

Fig. 2: Distinct roles for ClpP and ClpX in SOS response induction.

From: The ClpX protease is essential for inactivating the CI master repressor and completing prophage induction in Staphylococcus aureus

Fig. 2

Reporter plasmids were designed to place the β-lactamase reporter gene (blaZ) of plasmid pCN41 under the control of the SOS-controlled promoters of lexA or recA. RN4220 derivative strains containing the indicated plasmids were grown to exponential phase, split and the SOS response was induced in one half of the culture with MitC while the other half was left untreated. Samples were taken 90 min after induction. Fold induction change of MitC-induced against non-induced samples is shown. Bold horizontal lines in each boxplot represent the median and lower and upper hinges of the first and third quartiles, respectively (n = 3 biological replicates). Assessment of statistically significant differences between groups was performed using ANOVA followed by Tukey’s HSD post-test. p values are indicated above each comparison.

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