Fig. 4: Distinct roles for ClpP and ClpX in phage induction.
Plasmid pCN41-derived reporter plasmids were designed to place the β-lactamase reporter gene (blaZ) under the control of the Φ11 cro promoter. These plasmids also contained the genes encoding for either the Φ11 WT CI (cIWT), an SOS-insensitive CI mutant (cIG131E) or the post-cleavage N-terminal domain of CI alone (cIG131*). Strains containing the indicated plasmids were grown to exponential phase, split and the SOS response induced in one-half of the culture with MitC. Samples were taken 90 min after induction. Bold horizontal lines in each boxplot represent the median and lower and upper hinges of the first and third quartiles, respectively (n = 3 biological replicates). Assessment of statistically significant differences between groups was performed using ANOVA followed by Tukey’s HSD post-test. p values are indicated above each comparison.
