Fig. 5: The Φ11 CI N-terminal domain affects phage infection in the absence of either ClpP or ClpX. | Nature Communications

Fig. 5: The Φ11 CI N-terminal domain affects phage infection in the absence of either ClpP or ClpX.

From: The ClpX protease is essential for inactivating the CI master repressor and completing prophage induction in Staphylococcus aureus

Fig. 5

Genes encoding either the Φ11 CI WT (CIWT), an SOS-insensitive version (CIG131E) or only its post-cleavage N-terminal fragment (CIG131*) were cloned into the inducible expression plasmid pCN51 and introduced into the indicated strains. Expression from these plasmids was maintained throughout the experiment by the addition of 1 μM CdCl2 during both growth and phage titration. Lawns of the defined, exponential phage, RN450 derivative strains were prepared on PB plates supplemented with 1 μM CdCl2 to maintain CI expression and serial dilutions of Φ11 lysate spotted onto these lawns. Bold horizontal lines in each boxplot represent the median and lower and upper hinges of the first and third quartiles, respectively (n = 3 biological replicates). Assessment of statistically significant differences between groups was performed using ANOVA followed by Tukey’s HSD post-test. p values are indicated above each comparison.

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