Fig. 7: ClpP and ClpX alter spontaneous prophage induction rates.
The indicated RN450 prophage lysogens were grown for MitC induction but were left untreated to monitor spontaneous prophage induction. Plaque formation was assessed on a lawn of RN4220 and normalised per experiment relative to phage titres in the wt strain background. Bold horizontal lines in each boxplot represent the median and lower and upper hinges of the first and third quartiles, respectively (n = 3 biological replicates). Assessment of statistically significant differences between groups was performed using a two-sided Student’s t test on log10 transformed data assessing the hypothesis that phage titres were not different from wt (fold-change = 0). p values are indicated above the respective comparison.
