Fig. 3: 11p15.5 mosaic hepatocytes disrupt liver zonation architecture. | Nature Communications

Fig. 3: 11p15.5 mosaic hepatocytes disrupt liver zonation architecture.

From: Preneoplastic liver colonization by 11p15.5 altered mosaic cells in young children with hepatoblastoma

Fig. 3

a Frequence of IGF2 positive cells (none to many) and IGF2 intensity in metabolic zones (1 to 3) where i3 stands for highest intensity in 8 non-mosaic and 9 mosaic livers (11 and 10 analyzed slides respectively). Two-sided χ² for trend were performed. CV: central vein, PV: portal vein. b Number of central veins per µm² analyzed and glutamine synthetase staining intensity around CV in 8 non-mosaic and 9 mosaic livers (11 and 10 analyzed slides respectively). “s” indicates the number of slides analyzed and “p” the number of corresponding patients. Two-sided Wilcoxon and χ² for trend statistical tests were performed. c Representative RNAscope of IGF2 and immunostainings of glutamine synthetase in paraffin-embedded liver sections from a non-mosaic patient (#3163), a non-mosaic and a mosaic area from a patient with 11p15.5 locus mosaic cn-LOH (#3377). All IGF2 isoforms (P0 to P4 promoters) were considered in IGF2 singleplex and duplex RNAscope assay. Two slides from patient #3377 and one slide from patient #3163 were analyzed. Scale bars, 50 µM and 200 µM. Source data are provided as a Source Data file.

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