Fig. 2: Atp8b1IEC-KO mice (line #12) have shortened villi and lower expression of apical membrane protein in SI.
From: Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and steatohepatitis

Proximal and distal SI were excised from male Atp8b1IEC-KO mice (line #12) and littermate Atp8b1flox/flox mice at the newborn stage (n = 5) and at 4 weeks old (n = 5) and subjected to histological analysis. a H&E staining of SI section. b Quantification of villus length. Each symbol indicates values of 50 villi from 5 mice in each group. c–f IHC staining of apical membrane markers, NHE3 (c), ezrin (d), DPPIV (e), and pERM (f), and proteins localized to basolateral membrane, E-cadherin (c, d) and β-catenin (e, f), in SI section. g–l IHC staining of IEC markers, lysozyme (Paneth cells; g), chgA (endocrine cells; h), and muc2 (goblet cells; i), in SI sections. j–l Quantification of IEC positive for lysozyme (j), chgA (k), and muc2 (l) per villus. In each mouse, more than 25 villi were evaluated from three images. Each symbol indicates the values of 5 mice/group. In (a, c–f, g–i), representative images are shown. Scale bars: 100 μm. In (b, j–l), all data are presented as mean ± SEM. P values were calculated by two-tailed, unpaired Welch’s t test and indicated in the figures if less than 0.05.