Fig. 2: Effect of Lcn2 deficiency on mitochondrial dynamics. | Nature Communications

Fig. 2: Effect of Lcn2 deficiency on mitochondrial dynamics.

From: Lipocalin 2 regulates mitochondrial phospholipidome remodeling, dynamics, and function in brown adipose tissue in male mice

Fig. 2

Mitochondrial dynamics induced by fasting and refeeding cycle in differentiated brown adipocytes (a). The expression of proteins involved in mitochondrial fission and fusion in differentiated brown adipocytes during the fasting-refeeding cycle (b) and upon norepinephrine (NE) (1 μM) or CL316, 243 (CL) (1 μM) treatment for one hour (c). TEM analysis of mitochondrial morphology and quantification of mitochondrial size in brown adipose tissue of mice at room temperature (RT) or after 5 h of 4 °C cold exposure (d). The in vitro experiments in b and c were repeated independently 3 and 2 times, respectively. The average mitochondrial size was determined based on 402 and 392 mitochondria from WT and Lcn2 KO mice at room temperature, and 496 and 510 mitochondria from WT and Lcn2 KO mice after 5 hours of exposure to 4 °C cold temperature (n = 3). Results are presented as mean ± SEM. Statistical significance was analyzed by Student’s t-test; all tests were two-sided. Source data are provided as a Source data file. PA: palmitate acid; HG: high glucose; LD: lipid droplet.

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