Fig. 5: PlsB2smg displays 2-acyl-G3P acyltransferase activity. | Nature Communications

Fig. 5: PlsB2smg displays 2-acyl-G3P acyltransferase activity.

From: Acylation of glycerolipids in mycobacteria

Fig. 5

The products of PlsB2smg acyltransferase reactions using 1-hydroxy-2-palmitoyl-G3P as the acceptor substrate and C18:1-CoA or C18:0-CoA as acyl donors were analyzed by LC/MS. a The generation of sn-1 and sn-2 LPA acceptors from 1,2-dipalmitoyl-sn-G3P is as described in Fig. 4A. b Incubation of this LPA mixture with PlsB2smg in the presence of C18:1-CoA or C18:0-CoA yields PA products harboring C16:0/C18:0 or C16:0/C18:1 acyl chains. c Digestion of these PA products with phospholipase A2 yields sn-1 LPAs with C18:0 or C18:1 chains confirming that the PlsB2smg-mediated acyl transfer occurred at position sn-1 of 1-hydroxy-2-palmitoyl-G3P. sn-1 LPAs comigrate with authentic sn-1-LPA standards (red traces).

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