Fig. 6: Effect of overexpressing plsMsmg and plsB2smg on glycerolipid synthesis by E. coli membranes.
a Phospholipid synthesis by E. coli membranes prepared from control cells (Ctl; harboring an empty pET28a plasmid) and plsMsmg overexpressing cells (OE). Reaction mixtures were as described under Methods with [14C(U)]G3P as the radiolabeled acceptor substrate and C16:0-CoA as the acyl donor. At the indicated time points, reactions were terminated and the products analyzed by TLC in the solvent system CHCl3:CH3OH:H2O (65:25:4 by vol.). b Phospholipid synthesis by E. coli membranes prepared from control cells (Ctl; harboring an empty pET14b plasmid) and plsB2smg overexpressing cells (OE). Reaction mixtures were as described under Methods with [14C(U)]G3P as the radiolabeled acceptor substrate and C18:1-CoA as the acyl donor. At the indicated time points, reactions were terminated and the products analyzed as described above. The results shown are representative of two (PlsB2) to three (PlsM) independent experiments. LPA, lysophosphatidic acid; PA, phosphatidic acid; CL, cardiolipin; PG, phosphatidylglycerol; PE, phosphatidylethanolamine; MAG, monoacylglycerol; DAG, diacylglycerol. Source data are provided as a Source Data file.
