Fig. 3: MAP70-5 regulates microtubule arrays on pits.
From: Confined-microtubule assembly shapes three-dimensional cell wall structures in xylem vessels
a Cortical microtubules (pIRX3-EYFP-TUB6) in metaxylem vessel cells of wild type (WT) and map70-1 map70-5. The images are representative of five independent experiments. (b and c) Cortical microtubules (pIRX3-EYFP-TUB6) in metaxylem vessel cells of wild type b and map70-1 map70-5 c, and magnified images of the areas outlined by white boxes. Cell walls are labeled with PI. Yellow arrowheads indicate microtubules extending beyond the edge of pits. d Ratio of length of pit microtubules and pits in wild type and map70-1 map70-5. **p < 0.01; Student’s t-test (n = 100 pits). e EB1b (pXCP1:GFP-EB1b) in metaxylem vessel cells of wild type and map70-1 map70-5 plants. The images are representative of five independent experiments. f Projection of EB1b time-laps images. Time-lapse images were taken every 2 second. g Time-laps images of EB1b (green) around the secondary cell wall pits. The signals were overlayed with the projection of EB1b time-lapse images (magenta). h Angle of cortical microtubules in metaxylem vessel cells in wild type and map70-1 map70-5. ns, not significant; Student’s t-test (n = 50 pits). i Correlation between the angle of pits and cortical microtubules around pits of wild type and map70-1 map70-5 (n = 20 pits). Microtubule angle is the mean of two to five microtubules around each pit. Scale bars: 5 µm.
