Fig. 2: Deletion-inversion bi-allele of AtPROPEP8/7/4/5 induced by multiplexed CRISPR editing. | Nature Communications

Fig. 2: Deletion-inversion bi-allele of AtPROPEP8/7/4/5 induced by multiplexed CRISPR editing.

From: Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants

Fig. 2

a Diagram of a putative TAG locus for CRISPR-mediated deletion with gRNAs and genotyping primers indicated. TAG-L and TAG-R denote the two outermost genes at the TAG locus, respectively. b Diagram of the delinver bi-allele for AtPROPEP8/7/4/5 in the presumptive homozygous atpropep1-8 T2 mutant line #13-2. Primers used for PCR-based genotyping are shown. Fw, forward primer. Rev, reverse primer. t, truncated version. c PCR-based genotyping revealed the delinver genotype of AtPROPEP8/7/4/5 in atpropep1-8 #13-2. Experiments were repeated twice with similar results. d Sanger sequencing of PCR amplicons using co-aligned primers validated the genomic inversion between gRNA-Pep5 and gRNA-Pep8 induced breakpoints in atpropep1-8 #13-2. Black bold letters mark PAMs. Target sequences of gRNAs are underlined. Source data are provided as a Source Data file.

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