Fig. 3: Delinver mutations are prevalent in targeted TAG deletion by multiplexed CRISPR system in Arabidopsis. | Nature Communications

Fig. 3: Delinver mutations are prevalent in targeted TAG deletion by multiplexed CRISPR system in Arabidopsis.

From: Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants

Fig. 3

a PCR-based genotyping revealed the delinver genotype of AtMC7/6/5/4 in the atmc4-7 T1 mutant line #87. Primers used for PCR-based genotyping are shown. Fw, forward primer. Rev, reverse primer. b PCR-based genotyping revealed the delinver genotype of AtCHI1/2/3/4/5/6 in the atchi1-6 T1 mutant line #25. In a and b experiments were repeated twice with similar results. c, d Sanger sequencing of PCR amplicons using co-aligned primers validated the inversion of AtMC7/6/5/4 between gRNA-MC4 and gRNA-MC7 induced breakpoints in atmc4-7 #87 (c) and inversion of AtCHI1/2/3/4/5/6 between gRNA-CHI1 and gRNA-CHI6 induced breakpoints in atchi1-6 #25 (d). In c and d black bold letters mark PAMs and target sequences of gRNAs are underlined. Source data are provided as a Source Data file.

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