Table 1 Results of genotyping PCR and corresponding possible genotypes

From: Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants

Summary of genotyping PCR outcomes for different genotypes

PCR

WT

Heterozygous TAG deletion

Homozygous TAG deletion

Delinver bi-allele

 

Tier 1 (Fw1 + Rev1)

−a

+b

+

+

Standard genotyping

Tier 2 (Fw1 + Rev2)

+

+

−

−

Tier 3 (Rev1 + Rev2)

−

−

−

+

 
  1. aThe minus symbol indicates negative amplicons.
  2. bThe plus symbol indicates positive amplicons.
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