Table 2 Summary of deletion and delinver mutations mediated by different gRNA pairs at different TAG loci in Arabidopsis
TAGs | gRNAs | Deletion size (kb) | Transgenic plants | Plants with deletions | Deletion frequency (%) | Plants with delinvers | Delinver frequency (%) | Delinver/deletion ratio (%) |
|---|---|---|---|---|---|---|---|---|
AtMC7/6/5/4 | gRNA-MC7 gRNA-MC4 | 8.2 | 90 | 3 | 3.3 | 1 | 1.1 | 33.3 |
AtCHI1/2/3/4/5/6 | gRNA-CHI1 gRNA-CHI6 | 18.7 | 157 | 30 | 19.1 | 17 | 10.8 | 56.7 |
AT5G45240/ AtRPS4/ AtRRS1 | L1 + R1 | 18.4 | 147 | 113 | 76.9 | 83 | 56.5 | 73.5 |
L1 + R2 | 18.3 | 8 | 5 | 62.5 | 2 | 25.0 | 40.0 | |
L1 + R3 | 18.2 | 32 | 0 | 0 | 0 | 0 | 0 | |
L1 + R4 | 18.2 | 383 | 270 | 70.5 | 203 | 53.0 | 75.2 | |
L1 + R5 | 18.2 | 10 | 2 | 20.0 | 1 | 10.0 | 50.0 | |
L1 + R6 | 18.2 | 18 | 14 | 77.8 | 10 | 55.6 | 71.4 | |
L1 + R7 | 18.1 | 218 | 132 | 60.6 | 90 | 41.3 | 68.2 | |
L1 + R8 | 17.9 | 101 | 6 | 5.9 | 0 | 0 | 0 | |
L2 + R9 | 14.3 | 24 | 15 | 62.5 | 12 | 50.0 | 80.0 | |
L2 + R10 | 11.2 | 98 | 39 | 39.8 | 20 | 20.4 | 51.3 | |
L3 + R9 | 11 | 46 | 13 | 28.3 | 2 | 4.3 | 15.4 | |
L3 + R10 | 8 | 208 | 24 | 11.5 | 6 | 2.9 | 25.0 |
