Table 4 Summary of deletion and delinver mutations mediated by different gRNA pairs at different non-TAG loci in Arabidopsis

From: Hidden prevalence of deletion-inversion bi-alleles in CRISPR-mediated deletions of tandemly arrayed genes in plants

Non-TAGs

gRNAs

Deletion size (kb)

Transgenic plants

Plants with deletion

Deletion frequency (%)

Plants with delinver

Delinver frequency (%)

Delinver/deletion ratio (%)

AtRRS1/

AT5G45275/

AT5G45276/

AT5G45277/

AT5G45280/

AT5G45290

L4 + R3

18.2

19

1

5.3

0

0

0

L5 + R3

18.2

104

39

37.5

21

20.2

53.8

L4 + R4

18.2

92

14

15.2

6

6.5

42.9

L5 + R4

18.2

105

27

25.7

19

18.1

70.4

AT5G47980/ AtTHAD/ AtTHAH/ AtTHAS

L1 + R1

32

80

45

56.3

30

37.5

66.7

L2 + R2

32.3

95

7

7.4

3

3.2

42.9

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